Comparison of five commercial extraction kits for subsequent membrane protein profiling
نویسندگان
چکیده
منابع مشابه
Comparison of the utility of five commercial kits for extraction of DNA from Aspergillus fumigatus spores.
The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using A...
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Differences in the composition of the gut microbiota have been associated with a range of diseases using culture-independent methods. Reliable extraction of nucleic acid is a key step in identifying the composition of the faecal microbiota. Five widely used commercial deoxyribonucleic acid (DNA) extraction kits (QIAsymphony® Virus/Bacteria Midi Kit (kit QS), ZR Fecal DNA MiniPrep™ (kit Z), QIAa...
متن کاملComparison of five commercial DNA extraction kits for the recovery of Francisella tularensis DNA from spiked soil samples.
Francisella tularensis is the etiologic agent of the zoonotic disease tularemia and is thought to be maintained in the environment principally by various terrestrial and aquatic vertebrate animals. The organism is known to persist in water or mud for long periods of time and Francisella-specific DNA has been identified from water and soil. To gain a better understanding of the ecology and epide...
متن کاملComparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens.
We evaluated six commercially available DNA extraction kits for their ability to recover DNA from various dilutions of cytomegalovirus (CMV) added to four different specimens: bronchoalveolar lavage, cerebral spinal fluid, plasma, and whole blood. The kits evaluated included the Puregene DNA isolation kit (PG), Generation Capture Column kit, MasterPure DNA purification kit, IsoQuick nucleic aci...
متن کاملEvaluation of five commercial kits to detect dsDNA antibodies.
Experiments were performed to evaluate five commercial kit assays used for the detection of antibodies to dsDNA. The kits were compared using a performance index score as recommended by the guidelines of the European Committee for Clinical Laboratory Standards. The highest performance score was obtained using the radioimmunoassay from Immunodiagnostic Services Ltd, with the Amersham kit second,...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2009
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-009-9249-1